Part:BBa_K2184011

TAS2R38 rs10246939-PTC SNP taste

Includes a unique characterization of the composition of taste receptor alleles for the five different tastes. This part will be carried out for each subject. Characterization will be carried out using Taqman technology with the FLUIDIGM system

selected 15 SNPs (Single Nucleotide Polymorphism) in favor of this reaction. Each of the SNP’s that were selected enables identification of a certain taste out of the five known senses of taste, Using SNP segments – the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. Whenever a sample DNA is tested a presence of a specific allele is checked by one of the 15 SNP's. receiving a fluorescence signal due to the release of the PROBE and vice versa. 67 DNA samples were tested using this method . Each sample was tested to check the presence of alleles to identify different tastes in accordance to the sequence of the SNP.

usage and biology

engineered selected SNP (Single Nucleotide Polymorphism) in favor of this reaction. the SNP that were created enable identification of a certain taste out of the five known senses of taste, each of the tastes will change the variability that make it possible to identify the taste . This selected SNP contein to PTC taste- rs10246939

pic 1- Results of the students running the DNA and the DNA of the 15 SNP panel in Taqman reaction conditions on the Fluidigm chip. Wild Type (Taste SNPs) marked with VIC- green color Mutant (Non-taste SNPs) be marked with FAM- red color blue color (show with green and red color)- mean that there are population genetic polymorphism in the country.  

pic 2- the population genetic polymorphism in the country in the context of this SNP.

Functional Parameters

Using SNP segments – the Taqman reaction makes it possible to identify the presence of a specific allele tested for a specific taste. sample DNA is tested for the presence of a specific allele rs10246939. SNPs are screened using the Fluidigm BioMark apparatus (Fluidigm Corp.) with 15 Assay-specific TaqMan we will receive a fluorescence signal due to the release of a PROBE and or not. 67 DNA samples were tested using this method. Each sample was tested to check for the presence of alleles to identify different tastes in accordance to the sequence of the SNP ordered